サンプルパッドが検体を吸収しない場合
- 疎水性材料は検体の広がりを妨げることがあります。処理バッファーに適切な界面活性剤を加え、濡れ性を改善します。
- Excessive or fixed BSA may slow flow. Reassess the BSA level and combine with Tween-20 or similar additives.
- After pretreatment, sample pads should be fully dried and stored under controlled humidity to reduce batch variation.
コンジュゲートパッドが放出しない場合
- 不均一または凝集したコロイド金粒子は、不活性なコンジュゲートの原因となる場合があります。まず金ゾル品質と結合安定性を確認してください。
- Release buffer should balance surfactants, protein protectants and sugars to support conjugate rehydration and reduce glass fiber adsorption.
- Sugar spraying and drying parameters should be stable to avoid electrostatic adsorption and incomplete release.
偽陽性、高バックグラウンド、感度低下の対処方法
- Check whether gold conjugate blocking is sufficient, antibody concentration is appropriate, and buffer pH/salt levels are suitable.
- For high membrane background, evaluate dispense volume, blocking system, sample matrix interference and chromatography speed together.
- Sensitivity loss is often linked to moisture content, foil pouch sealing, desiccant status and storage temperature; accelerated stability testing is recommended.
乾燥中にストリップが紫色または灰色になる理由
- Color change usually indicates colloidal gold aggregation or activity loss. Review drying temperature, humidity and time first.
- Over-drying may damage protein conformation, while insufficient drying affects long-term stability. A repeatable drying window is essential.
- 用途 pilot-scale matrices to validate temperature and humidity combinations, then confirm with flow, background and stability data.
Safhealは、サンプルパッド、コンジュゲート放出、偽陽性、感度、乾燥安定性など、コロイド金ラテラルフローテストストリップ製造の一般的な課題を整理しています。